Multicytokine production of CD4+ and CD8+ effectors in the peripheral blood (PBMC) or infiltrating the tumor (TIL) of a patient with renal cell carcinoma. T cells producing IL-10 are highlighted in black on IFN-γ+ and/or TNF-α+ effectors (Polychromatic flow cytometry, Attig et al, Cancer Res 2009;69:8412).
Induction of vaccine-specific CD4+ T cells in one patient with prostate carcinoma after peptide-based vaccination (IFN-γ ELISPOT assay with HLA-class II-binding peptides).
Phenotypic characterisation of vaccine induced CD8+ T cells using HLA-multimers and differentiation markers (Polychromatic flow cytometry)
Identification of antigen-specific CD8+ T cells. Production of cytokine (IFN-γ and TNF-α) in primed healthy donor CD8+ T lymphocytes after stimulation with two HLA-A*0201-restricted peptides (Polychromatic flow cytometry)
VITAL assay to assess the cytotoxic potential of tumor antigen specific CD8+ clones against peptide-loaded targets or trasnfectants expressing the relevant tumor antigen (Polychromatic flow cytometry, Laske et al, Cancer Immunol Res. 2013;190-200)