Groups
The lab is well-versed in the following molecular biology, cell biological and immunological techniques:
- Gateway Ligation-independent cloning
- LUMIER interaction screening
- Reporter assays, e.g. dual luciferase NF-kB activation
- Bioluminescence resonance energy transfer (BRET) for intramolecular conformation analysis or intermolecular interaction questions in living cells
- RT qPCR
- SNP genotyping and mutation analysis
- Immunoprecipitation and immunoblot
- WES (ProteinSimple) quantitative immuno-protein analysis
- Live cell confocal microscopy
- Immunofluorescence of human and mouse tissues
- ELISA
- Flow cytometry (including ICS)
- Lentiviral transduction
- Isolation, manipulation and functional analysis of primary human monocytes, macrophages, B cells, neutrophils and keratinocytes
- Whole blood assays
- In vivo experiments